Hi Barb,
It is my understanding that because flow cytometry is automated, they can quickly analyze 1000's of cells. However, with manual counts, they may only analyze 100's of cells.
As to why the numbers are so far off, I can only speculate. Perhaps the manual count is high because they were counting all blast cells, whereas the flow cytometry may have only been specifically looking for those with CD34?? Or perhaps the flow cytometry machine was not calibrated correctly??? I am just guessing here. Please let us know what you learn
Have you started or will you be starting Revlimid?
Hope your BMB results are stable!